IP Journal of Urology, Nephrology and Hepatology Science

Elevation of free prostate - specific antigen (FPSA) in cancer patients adenosine deaminase (ADA) Binds and stimulates plasminogen (PG) activation on 1-LN Human prostate cancer cells

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Article Type : Original Article

Author Details: M.A. Majid Adil,Shaikh Mahmood*,Mohd. Abbas Hyder

Volume : 4

Issue : 4

Online ISSN : XXXX

Print ISSN : XXXX

Article First Page : 109

Article End Page : 112

Abstract

The Prostate Specific Antigen (PSA) is the best and most commonly used tumor maker for prostate in clinical practice these days. 1-2 It is a glycoprotein has molecular weight of 33KD. PSA is very routinely used in the diagnosis and follow up of prostate disease, specially prostate cancer. 3-4 Prostate Specific Antigen (PSA) is formed in two different molecular types in serum; they are free PSA (fPSA) and complex PSA (cPSA), the serum of which form the total PSA (tPSA).fPSA is mostly used in the detection of Prostate cancer. 5 Free PSA 28 kda is eliminated by glomerular filtration. The date revealed that the patients with end stage of renal dysfunction prolonged dialysis with increase percent of fPSA, 6-9

Introduction: Adenosine Deaminase (ADA) is expressed in intracellular by all the cells. It is also associated with the cell surface multifunctional. Glycoprotein CD26/ dipeptidyl peptidase IV. CD26 is expressed on the surface of human prostate cancer. 1-LN cells acting as a receptor for plasminogen (pg). Since ADA and pg bind CD26 of distinct nearby sites. A possible interaction is found between these two proteins on the surface of 1-LN cells. Human ADA binds to CD26 on the surface 1-LN cells. Thus PSA, ADA may be factors regulating events in prostate cancer cells that occur when pg binds to the cell surface and in activated in prostate cancer, 10-13

Materials and Methods: A total number of 201 patients with age group of (Median age 58 years, interquartile age range 47-68) with Chronic Kidney Disease (CKD). Their Glomerular Filtration Rate (GFR) was 24 ml/min/1.7cm2 . (Interquartile range is 18-34) by determined by iohexol clearance method. A separate biochemical and quantitative estimation was done for serum Adenosine Deamine (ADA) and Plasminogen (pg) was done by fluroimmunoessay method and are found elevated as serum Adenosine Deamine (ADA) > 10Iu/L and plasminogen (pg) > 0.2 Iu/L in prostate cancer patients. 14-17 Control included 350 subjects, attended a positive cancer screening test with no diagnostic prostate cancer.

Results: Mediant PSA levels and percent of f PSA were significantly higher (p<0>

Conclusion: The present study of percent of free Prostate Specific Antigen (fPSA) Adenosine Deamine (ADA) on plasminogen (pg) are significantly influenced by moderately impaired renal function in patients with Chronic Kidney Disease (CKD) and in prostate cancer.

Keywords: Prostate specific antigen (PSA), Adenosine deaminase (ADA), Plasminogen (pg), CD26, 1-LN Cells, Chronic kidney disease (CKD), Glomerular filtration rate (GFR) and prostatic cancer

Doi No:-10.18231