Panacea Journal of Medical Sciences

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Creatine Kinase-MB measurement using Immunoinhibition methodology. How much is the interference?

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Original Article

Author Details : Rashmi Gulia*, Binita Goswami

Volume : 10, Issue : 2, Year : 2020

Article Page : 114-119

https://10.18231/j.pjms.2020.026

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Abstract

Introduction: Creatine kinase-MB (CK-MB) is considered as sensitive and specific indicator for
myocardial infarction. Various methods are available for CK-MB measurement but are associated
with inherent shortcomings. The routinely used Immunoinhibition method is evaluated for the various
interferents and their impact on the estimated CK-MB value.
Materials and Methods: The CK-MB values were analyzed for 500 patient samples whose total CK
values were within the normal reference interval. Immunoinhibition method (INH) & N-Acetylcysteine
(NAC) methods were used for CK-MB and total CK estimation respectively.
Results: The observed mean S.D. for CK was 90.15 48.9 U/L and for CK-MB was 33.29 20.47
U/L. The box and whisker plots were used to represent the data of CK & CK-MB. The CK-MB levels were
observed to exceed the total CK under certain circumstances owing to interferents. The CK-MB values
were observed to exceed the total CK in 8.6% of the cases.
Conclusion: The utility of CK-MB has ever been questioned. It has been categorized as over-used test.
The conventional immunoassay methodology for CK-MB measurement is not free from interferences,
awareness about these is important while interpreting the results so as to exclude method related artifactual
values.

Keywords: Creatine kinase, CKMB, Immunoinhibition, Assay interference, Macro- creatine kinase.

How to cite : Gulia R , Goswami B, Creatine Kinase-MB measurement using Immunoinhibition methodology. How much is the interference?. Panacea J Med Sci 2020;10(2):114-119

Copyright © 2020 by author(s) and Panacea J Med Sci. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org)