Panacea Journal of Medical Sciences
Official Publication of NKP Salve Institute of Medical Sciences and Research Centre
Official Publication of NKP Salve Institute of Medical Sciences and Research Centre
Print ISSN:-2249-8176
Online ISSN:-2348-7682
CODEN : PJMSD7
Current Issue
Year 2024
Volume: 14 , Issue: 2
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Original Article
Author Details :
Volume : 14, Issue : 1, Year : 2024
Article Page : 63-69
https://10.18231/j.pjms.2024.013
Abstract
Introduction: The present study was aimed at the molecular detection of HBV infection among patients with chronic liver disease (CLD) and to study the mutation spectrum S-gene region in the occult HBV strains.
Materials and Methods: An observational study conducted on 200 clinically diagnosed CLD cases enrolled from the Gastroenterology unit. Two hundred serum samples were screened with Fibroscan and for HBsAg, HBCT and estimated liver enzymes. Samples positive for either HBsAg or Anti HBc total were further evaluated for other HBV markers and presence of HBV DNA. The S region was amplified in occult CHB cases and sequenced.
Results: 70.5% (141) of the patients with CLD were suffering with Chronic Hepatitis B virus (CHB). The HBV DNA was detected in 19.14% patients. Four cases were in occult phase. Two samples of them could be amplified. The isolates belong to the genotype A, and revealed certain mutations leading to novel amino acids.
Conclusions: Mutations in the S gene sequences of HBV virus from the Occult HBV infection (OBI) patients would lead to immune escape or cause occult infection. In the Genotype A at two positions, amino acids that are likely to cause occult infection are observed apart from mutations at certain positions leading to novel amino acids.
Keywords: Chronic HBV infection, Occult HBV, Molecular diagnosis of HBV, Sgene
How to cite : Kolli P, Murty D S, Jyothi P, Metta M, Kavitha A, Lokeshu T, Cross sectional study on the molecular detection of Hepatitis B virus in patients with chronic liver disease in Guntur, India revealed mutations in Genotype A of occult HBV. Panacea J Med Sci 2024;14(1):63-69
Copyright © 2024 by author(s) and Panacea J Med Sci. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org)
