Panacea Journal of Medical Sciences

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Bacteriological profile of diabetic foot ulcer

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Original Article

Author Details : Shwetha DC*, Venkatesha D

Volume : 14, Issue : 2, Year : 2024

Article Page : 425-429

https://10.18231/j.pjms.2024.076

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Abstract

Introduction: Infected Diabetic foot ulcer is the common cause of hospitalization among patients with diabetes mellitus. Amputation of the lower limbs is the most common complication of Diabetic foot ulcer & infection is the cause in majority of the cases. Hence, Characterizing the bacteriological profile & antibiotic susceptibility testing is very important for the effective management of Diabetic foot ulcer.
Materials and Methods: Swabs from infected Diabetic foot ulcer were inoculated on to Blood agar & MacConkey agar. The bacterial identification was done according to standard protocol. The Antibiotic susceptibility testing for the isolated bacteria was done on Mueller Hinton agar by Kirby Bauer’s Disc Diffusion method.
Results: 134 aerobic bacteria were isolated from 110 diabetic patients. Among Gram positive cocci, (11.9%) was the predominant isolate followed by Coagulase negative Staphylococci (4.5%) & spp (7.5%). (18.6%) was the predominantly isolated Gram-negative bacilli followed by Klebsiella spp (17.1%), spp (11.2%), Pseudomonas spp (7.5%), spp (7.5%), spp (7.5%), Non-fermenting Gram negative bacilli (5.2%) & spp (1.5%). We found variations in the antibiotic sensitivity of isolated pathogens. 13.7% of Gram-negative bacilli were Extended Spectrum Beta Lactamase (ESBL) producers & 31.2% of Staphylococci were Methicillin Resistant Staphylococcus aureus (MRSA).
Conclusion: Our study shows that the repeated bacteriological evaluation & their antibiotic susceptibility is paramount in the management of infected Diabetic foot ulcer & the abuse of antibiotics should be restrained to prevent the emergence of antibiotic resistance.
 

Keywords: Diabetic foot ulcer, E. coli, Staphylococcus aureus, Antibiotic sensitivity testing

How to cite : Shwetha Dc, Venkatesha D, Bacteriological profile of diabetic foot ulcer. Panacea J Med Sci 2024;14(2):425-429

Copyright © 2024 by author(s) and Panacea J Med Sci. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org)