The Pharmacist
The Pharmacist (Pharmacist) is an open-access, peer-reviewed pharmacy journal, published half-yearly, as print and online by the The Pharmacist (Pharmacist) since 2025. With the aim of faster and better dissemination of knowledge, we will be publishing articles ‘Ahead of Print’ immediately upon acceptance of manuscript. In addition, the journal allows free access (Open Access) to its contents, which is likely to attract more readers and citations to articles published in journal. Manuscripts should be prepared in accordance w...
RP HPLC method development & validation for vadadustat in bulk drug and pharmaceutical dosage forms
Page: 47–52
Vadadustat was quantitatively estimated in bulk and dose forms using a quick, stability-indicating reverse-phase high-performance liquid chromatographic (RP-HPLC) method that was developed and validated. With a flow rate of 1.0 mL/min, an isocratic mobile phase consisting of acetonitrile and 0.1% triethylamine buffer (pH 2.5) (30:70 v/v), and UV detection at 234 nm, the best separation was accomplished on an Agilent Eclipse XDB C18 column. A 2.563-minute retention time was displayed by Vadadustat. ICH-recommended validation of the method showed strong precision, high accuracy (99.4–101.1% recovery), and excellent linearity (R 2 = 0.9998 in the range of 37.5−225 μg/mL). Studies on forced deterioration validated the stability-indicating character of the approach. Vadadustat quality control can be done easily, reliably, and with the established RP-HPLC method.
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- DOI 10.18231/j.pharmacist.45549.1765197099
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- Citation
- Received Date September 10, 2025
- Accepted Date October 09, 2025
- Publication Date December 09, 2025